Racing-Thoroughbreds: Plasma samples were taken from 178 healthy, fit, actively competing Thoroughbred Chuckwagon racing geldings (ages 4–18; median 9 years) when the horses were at rest in the morning prior to races. This interpretation is unlikely, and indicates caution must be used when applying this cut-off point to various horse populations. The predefined master curve is adapted to the analyzer using the relevant CalSet. All included patients provided written informed consent to the participation in the study. Telemetric ECG was subsequently run stall-side intermittently over 48 h. Plasma hscTnT analyte levels were measured at 5 and 72 h following the event. The use of only 20 horses in the reference population used by Van Der Vekens et. al.  led to a “tight” reference range, and significant overlap between their normal group and those classified with primary myocardial disease.
However, 391 ng/L is > 10 times our defined URLs, so linearity to this level should confer good diagnostic sensitivity for distinguishing sick from healthy populations. The horse was diagnosed stall-side, via auscultation, with ventricular tachycardia immediately following racing. Including a minimum of 120 horses in each of our groups provided populations for which reference intervals could be defined by statistical methods in compliance with ASCVP guidelines and provide better whole population normal hscTnT level estimations. The gelding later presented with near-collapse event at the end of his race secondary to an acute upper airway obstruction. This assay can be used as an aid in the differential diagnosis of acute coronary syndrome to identify necrosis, e.g. acute myocardial infarction. When several measurements were done, post bronchodilatation measurements prior to inclusion were preferred.
Examination showed cyanotic mucous membranes and persistent tachycardia (heart rate: 180 bpm). No cardiac murmurs were noted on auscultation. The analyzer was calibrated using human cTnT standards supplied for use of the hscTnT assay kit with human plasma. The application allows the monitoring of the systems, alarms and reagent information. Linear dose response N = 10 dilutions were prepared from purified equine cardiac troponin stock by serial 1:2 dilution (from 1:100 to 1:0.2) using normal equine plasma (hscTnT 3.28 ng/L) as diluent. Gender was kept in the model by convention.